2009-04-30
A Mass spectrometry based imaging method developed for the intracellular detection of HIV protease inhibitors
Publication
Publication
Rapid Communications in Mass Spectrometry , Volume 23 - Issue 8 p. 1183- 1188
Mass spectrometry imaging is a promising technique for measuring drugs and drug metabolites in cells and tissues. In this manuscript we describe a method for the imaging of HIV protease inhibitors. As a model system we used Mono Mac 6 cells cultured with the HIV protease inhibitors saquinavir and nelfinavir deposited on glass slides using a cytocentrifuge. A sublimation/deposition device for homogeneous matrix deposition was constructed which allows imaging of these HIV protease inhibitors at clinically relevant concentrations. Using this matrix sublimation/deposition method, glass slides containing the cytocentrifuged cells can be measured and analyzed by two types of mass spectrometry techniques, viz. matrix-assisted laser desorption/ionization time-of-flght (MALDI-TOF) and MALDI Fourier transform ion cyclotron resonance (FTICR), and this makes it possible to perform imaging rapidly (MALDI-TOF) and with a very high selectivity (MALDI-FTICR). Copyright
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doi.org/10.1002/rcm.3981, hdl.handle.net/1765/24123 | |
Rapid Communications in Mass Spectrometry | |
Organisation | Erasmus MC: University Medical Center Rotterdam |
Dekker, L., Kampert, J. J. A. V., Reedijk, M., Burgers, P., Gruters, R., Osterhaus, A., & Lyider, T. (2009). A Mass spectrometry based imaging method developed for the intracellular detection of HIV protease inhibitors. Rapid Communications in Mass Spectrometry, 23(8), 1183–1188. doi:10.1002/rcm.3981 |