Ectopic expression of Thy-1 in the kidneys of transgenic mice induces functional and proliferative abnormalities.
Hybrid human--mouse Thy-1.1 genes were injected into pronuclei of Thy-1.2 mice to produce transgenic animals. A hybrid gene composed of the 5' part of the mouse Thy-1.1 gene combined with the 3' human untranslated regions was expressed abnormally in the kidney podocytes, which resulted in severe protein-uria and subsequent death in several founder mice. A hybrid Thy-1 gene composed of the human coding region with the 5' and 3' flanking regions of the mouse gene was expressed abnormally in a different part of the kidney (the tubular epithelia), which resulted in a proliferative kidney disorder. In addition, a neoplasm was found in the brain of one of these mice. These results show that the Thy-1 protein can play an important role in the activation, proliferation, and differentiation of many different cell types.
|Keywords||*Gene Expression Regulation, 0 (Antigens, Surface), 0 (Antigens, Thy-1), 0 (Isoantibodies), 0 (anti-Thy antibody), Animals, Antigens, Surface/*genetics/immunology, Antigens, Thy-1, Cell Division, Cells, Cultured, Epithelium/immunology/pathology, Human, Isoantibodies/analysis, Kidney Diseases/genetics/*immunology/pathology, Kidney Glomerulus/immunology/pathology, Kidney Tubules, Proximal/immunology/pathology, Kidney/*immunology/pathology, Mice, Mice, Inbred C57BL, Mice, Inbred CBA, Mice, Transgenic, Proteinuria/immunology, Support, Non-U.S. Gov't|
Kollias, G., Evans, D.M., Ritter, M., Beech, J., Morris, R., & Grosveld, F.G.. (1987). Ectopic expression of Thy-1 in the kidneys of transgenic mice induces functional and proliferative abnormalities.. Cell, 51, 21–31. Retrieved from http://hdl.handle.net/1765/2423