Functional Analysis of the Human Neurofilament Light Chain Gene Promoter
We have carried out a structural and functional analysis on the human NF-L (H-NF-L) gene. It contains a methylation-free island, spanning the 5' flanking sequences and the first exon and a number of neuronal-specific DNase I hypersensitive sites have been identified in the upstream region as well as within the body of the gene. Analysis in cell lines and transgenic mice using a combination of these sites has revealed the presence of a conserved element(s) between -300bp and -190bp which is required for neuronal-specific expression.
|Keywords||*Promoter Regions (Genetics), 0 (Chromatin), 0 (Neurofilament Proteins), 0 (neurofilament protein L), 9007-49-2 (dna), Animals, Base Sequence, Cell Line, Chromatin/ultrastructure, Deoxyribonuclease I, Dna, EC 184.108.40.206 (Deoxyribonuclease I), Gene Expression, Hela Cells, Human, Mice, Molecular Sequence Data, Neurofilament Proteins/*genetics/metabolism, Restriction Mapping, Sequence Homology, Nucleic Acid, Support, Non-U.S. Gov't, Transfection|
Yazdanbakhsh, K., Fraser, P., Kioussis, D., Vidal, M., Grosveld, F.G., & Lindenbaum, M.H.. (1993). Functional Analysis of the Human Neurofilament Light Chain Gene Promoter. Nucleic Acids Research, 21, 455–461. Retrieved from http://hdl.handle.net/1765/2487