Embryonic expression and cloning of the murine GATA-3 gene.
We describe the embryonic expression pattern as well as the cloning and initial transcriptional regulatory analysis of the murine (m) GATA-3 gene. In situ hybridization shows that mGATA-3 mRNA accumulation is temporally and spatially regulated during early development: although found most abundantly in the placenta prior to 10 days of embryogenesis, mGATA-3 expression becomes restricted to specific cells within the embryonic central nervous system (in the mesencephalon, diencephalon, pons and inner ear) later in gestation. GATA-3 also shows a restricted expression pattern in the peripheral nervous system, including terminally differentiating cells in the cranial and sympathetic ganglia. In addition to this distinct pattern in the nervous system, mGATA-3 is also expressed in the embryonic kidney and the thymic rudiment, and further analysis showed that it is expressed throughout T lymphocyte differentiation. To begin to investigate how this complex gene expression pattern is elicited, cloning and transcriptional regulatory analyses of the mGATA-3 gene were initiated. At least two regulatory elements (one positive and one negative) appear to be required for appropriate tissue-restricted regulation after transfection of mGATA-3-directed reporter genes into cells that naturally express GATA-3 (T lymphocytes and neuroblastoma cells). Furthermore, this same region of the locus confers developmentally appropriate expression in transgenic mice, but only in a subset of the tissues that naturally express the gene.
|Keywords||0 (DNA-Binding Proteins), 0 (GATA-3 factors), 0 (Trans-Activators), 0 (Transcription Factors), Animals, Base Sequence, DNA-Binding Proteins/*genetics, Gene Expression, Gene Expression Regulation, In Situ Hybridization, Kidney/*embryology, Mice, Mice, Inbred BALB C, Mice, Transgenic, Molecular Sequence Data, Nervous System/*embryology, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S., Trans-Activators/*genetics, Transcription Factors/*genetics, Transcription, Genetic, Zinc Fingers/*genetics|
George, K.M., Leonard, M.W., Roth, M.E., Lieuw, K.H., Kioussis, D., Grosveld, F.G., & Engel, J.D.. (1994). Embryonic expression and cloning of the murine GATA-3 gene.. Development (Cambridge), 120, 2673–2686. Retrieved from http://hdl.handle.net/1765/2508