To examine the role of the Oct-6 gene in Schwann cell differentiation we have cloned and characterized the chicken and zebrafish homologues of the mouse Oct-6 gene. While highly homologous in the Pit1-Oct1/2-Unc86 (POU) domain, sequence similarities are limited outside this domain. Both genes are intronless and both proteins lack the amino acid repeats that are a characteristic feature of the mammalian Oct-6 proteins. However as in mammals, the aminoterminal parts of the chicken and zebrafish Oct-6 proteins are essential for transactivation of octamer containing promoters. By immunohistochemistry we have found that the chicken Oct-6 protein is expressed in late embryonic ensheathing Schwann cells of the sciatic nerve and is rapidly downregulated when myelination proceeds. This expression profile in glial cells is identical to that in the mouse and rat. Furthermore the zebrafish Oct-6 homolog is expressed in the posterior lateral nerve at a time when it contains actively myelinating Schwann cells. Thus despite extensive primary sequence divergence among the vertebrate Oct-6 proteins, the expression of the chicken and zebrafish Oct-6 proteins is consistent with the notion that Oct-6 functions as a 'competence factor' in promyelin cells to execute the myelination program.

Additional Metadata
Keywords HeLa cell, POU3F1 protein, Pou3f1 protein, Schwann cell, amino acid sequence, animal, animal anatomy, article, chemistry, chicken, comparative study, embryo, embryo development, gene expression regulation, gene function, gene library, gene sequence, genetic analysis, genetic transfection, genetics, human, immunohistochemistry, innervation, metabolism, molecular cloning, molecular evolution, molecular genetics, mouse, myelin sheath, nerve protein, nonhuman, octamer transcription factor 6, open reading frame, peripheral nerve, physiology, pou50 protein, prenatal development, priority journal, protein expression, protein tertiary structure, species difference, structure activity relation, transactivation, transcription factor, transcription factor POU, zebra fish, zebrafish, zebrafish protein
Persistent URL dx.doi.org/10.1016/S0925-4773(98)00067-7, hdl.handle.net/1765/2562
Citation
Levavasseur, F., Mandemakers, W.J., Visser, P., Broos, L., Grosveld, F.G., Zivkovic, D., & Meijer, D.N.. (1998). Comparison of sequence and function of the Oct-6 genes in zebrafish, chicken and mouse.. Mechanisms of Development, 74(1-2), 89–98. doi:10.1016/S0925-4773(98)00067-7