Transposition of the Drosophila hydei Minos transposon in the mouse germ line.
We tested the suitability of the fly transposon Minos, a member of the Tc1/mariner superfamily, for insertional mutagenesis in the mouse germ line. We generated a transgenic mouse line expressing Minos transposase in growing oocytes and another carrying a tandem array of nonautonomous transposons. The frequency of transposition in the progeny derived from oocytes carrying both transgenes is 8.2%. Analysis of the new integration sites shows a high frequency of transpositions to a different chromosome. Thus Minos transposition could be an effective system for insertional mutagenesis and functional genomic analysis in the mouse.
|Keywords||*DNA Transposable Elements, Animals, Blotting, Southern, Drosophila/*genetics, In Situ Hybridization, Fluorescence, Mice|
|Persistent URL||dx.doi.org/10.1016/S0888-7543(02)00030-7, hdl.handle.net/1765/2631|
Drabek, D.D., Zagoraiou, L., Langeveld, A., Roumpaki, C., Mamalaki, C., Savakis, C., … de Wit, T.P.M.. (2003). Transposition of the Drosophila hydei Minos transposon in the mouse germ line.. Genomics, 81(2), 108–111. doi:10.1016/S0888-7543(02)00030-7