2002-09-02
A cell type-specific allele of the POU gene Oct-6 reveals Schwann cell autonomous function in nerve development and regeneration.
Publication
Publication
EMBO Journal , Volume 21 - Issue 17 p. 4612- 4620
While an important role for the POU domain transcription factor Oct-6 in the developing peripheral nerve has been well established, studies into its exact role in nerve development and regeneration have been hampered by the high mortality rate of newborn Oct-6 mutant animals. In this study we have generated a Schwann cell-specific Oct-6 allele through deletion of the Schwann cell-specific enhancer element (SCE) in the Oct-6 locus. Analysis of mice homozygous for this allele (ΔSCE allele) reveals that rate-limiting levels of Oct-6 in Schwann cells are dependent on the SCE and that this element does not contribute to Oct-6 regulation in other cell types. We demonstrate a Schwann cell autonomous function for Oct-6 during nerve development as well as in regenerating nerve. Additionally, we show that Krox-20, an important regulatory target of Oct-6 in Schwann cells, is activated, with delayed kinetics, through an Oct-6-independent mechanism in these mice.
Additional Metadata | |
---|---|
, , , , , , , , , , , , , , , , , , , , | |
doi.org/10.1093/emboj/cdf475, hdl.handle.net/1765/2635 | |
EMBO Journal | |
Organisation | Erasmus MC: University Medical Center Rotterdam |
Ghazvini, M., Mandemakers, W., Jaegle, M., Piirsoo, M., Koutsourakis, M., Smit, X., … Driegen, S. (2002). A cell type-specific allele of the POU gene Oct-6 reveals Schwann cell autonomous function in nerve development and regeneration. EMBO Journal, 21(17), 4612–4620. doi:10.1093/emboj/cdf475 |