Despite the mitochondria ubiquitous nature many of their components display divergences in their expression profile across different tissues. Using the bioinformatics-approach of guilt by association (GBA) we exploited these variations to predict the function of two so far poorly annotated genes: Coiled-coil-helix-coiled-coil-helix domain containing 10 (CHCHD10) and glioblastoma amplified sequence (GBAS). We predicted both genes to be involved in oxidative phosphorylation. Through in vitro experiments using gene-knockdown we could indeed confirm this and furthermore we asserted CHCHD10 to play a role in complex IV activity.

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Keywords CHCHD10, Complex IV, Functional prediction, GBAS, Oxidative phosphorylation
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Martherus, R.S.R.M., Sluiter, W., Timmer, E.D.J., VanHerle, S.J.V., Smeets, H.J., & Ayoubi, T.A.Y.. (2010). Functional annotation of heart enriched mitochondrial genes GBAS and CHCHD10 through guilt by association. Biochemical and Biophysical Research Communications, 402(2), 203–208. doi:10.1016/j.bbrc.2010.09.109