Induction of progressive demyelinating autoimmune encephalomyelitis in common marmoset monkeys using MOG34-56 peptide in incomplete freund adjuvant
Experimental autoimmune encephalomyelitis in the neotropical primate common marmoset (Callithrix jacchus) is a relevant autoimmune animal model of multiple sclerosis. T cells specific for peptide 34 to 56 of myelin/oligodendrocyte glycoprotein (MOG34-56) have a central pathogenic role in this model. The aim of this study was to assess the requirement for innate immune stimulation for activation of this core pathogenic autoimmune mechanism. Marmoset monkeys were sensitized against synthetic MOG34-56 peptide alone or in combination with the nonencephalitogenic peptide MOG74-96 formulated in incomplete Freund adjuvant, which lacks microbial components. Experimental autoimmune encephalomyelitis development was recorded by monitoring neurological signs, brain magnetic resonance imaging, and longitudinal profiling of cellular and humoral immune parameters. All monkeys developed autoimmune inflammatory/demyelinating central nervous system disease characterized by massive brain and spinal cord demyelinating white matter lesions with activated macrophages and CD3 T cells. Immune profiling ex vivo demonstrated the activation of mainly CD3CD4/8CD56 T cells against MOG34-56. Upon ex vivo stimulation, these T cells produced more interleukin 17A compared with TH1 cytokines (e.g. interferon-γ) and displayed peptide-specific cytolytic activity. These results indicate that the full spectrum of marmoset experimental autoimmune encephalomyelitis can be induced by sensitization against a single MOG peptide in incomplete Freund adjuvant lacking microbial compounds for innate immune activation and by eliciting antigen-specific T-cell cytolytic activity.
|Keywords||17, Cytotoxicity, Experimental autoimmune encephalomyelitis, H, Innate immunity, Multiple sclerosis, Myelin oligodendrocyte glycoprotein, Nodlike receptor, Pattern recognition receptor, T, Toll like receptor|
|Persistent URL||dx.doi.org/10.1097/NEN.0b013e3181d5d053, hdl.handle.net/1765/27549|
Jagessar, S.A., Kap, Y.S., Heijmans, N., van Driel, N., van Straalen, L., Bajramovic, J.J., … 't Hart, B.. (2010). Induction of progressive demyelinating autoimmune encephalomyelitis in common marmoset monkeys using MOG34-56 peptide in incomplete freund adjuvant. Journal of Neuropathology and Experimental Neurology, 69(4), 372–385. doi:10.1097/NEN.0b013e3181d5d053