Hepatitis B vaccine-specific CD4+ T cells can be detected and characterised at the single cell level: Limited usefulness of dendritic cells as signal enhancers
Hepatitis B surface antigen (HBsAg)-specific T cells were analysed in 16 healthy individuals vaccinated against hepatitis B, using optimised protocols. HBsAg-specific IFN-γ producing CD4+T cells were found at a similar low frequency (0.01%) within the naive T cell subset, the central and the effector memory T cell subset. Overall, HBsAg-specific total CD4+T cells were detected in approximately 81% of the HBV vaccinees. The use of dendritic cells substantially increased the otherwise low proliferative responses but not the percentage of IFN-γ producing cells. The analysis of readily detectable CMV-specific CD4+T cell responses was used as a positive control and confirmed the adequacy of our assays. T cell responses associated with (in-) adequate hepatitis B vaccination can now be analysed in more detail.
|Keywords||Antigen-specific T cells, Cytokine flow cytometry, Dendritic cells, Hepatitis B|
|Persistent URL||dx.doi.org/10.1016/j.jim.2007.10.013, hdl.handle.net/1765/28744|
|Journal||Journal of Immunological Methods|
Litjens, N.H.R, Huisman, M, Baan, C.C, van Druningen, C.J, & Betjes, M.G.H. (2008). Hepatitis B vaccine-specific CD4+ T cells can be detected and characterised at the single cell level: Limited usefulness of dendritic cells as signal enhancers. Journal of Immunological Methods, 330(1-2), 1–11. doi:10.1016/j.jim.2007.10.013