High EVI1 levels predict adverse outcome in acute myeloid leukemia: Prevalence of EVI1 overexpression and chromosome 3q26 abnormalities underestimated
Inappropriate expression of EVI1 (ecotropic virus integration-1), in particular splice form EVI1-1D, through chromosome 3q26 lesions or other mechanisms has been implicated in the development of high-risk acute myeloid leukemia (AML). To validate the clinical relevance of EVI1-1D, as well as of the other EVI1 splice forms and the related MDS1/EVI1 (ME) gene, real-time quantitative polymerase chain reaction was performed in 534 untreated adults with de novo AML. EVI1-1D was highly expressed in 6% of cases (n = 32), whereas 7.8% were EVI1+(n = 41) when all splice variants were taken into account. High EVI1 predicted a distinctly worse event-free survival (HR = 1.9; P = .002) and disease-free survival (HR = 2.1, P = .006) following multivariate analysis. Importantly, we distinguished a subset of EVI1+cases that lacked expression of ME (EVI1+ME-; n = 17) from cases that were ME+(EVI1+ME+; n = 24). The atypical EVI1+ME-expression pattern exhibited cytogenetically detectable chromosomal 3q26 breakpoints in 8 cases. Fluorescence in situ hybridization revealed 7 more EVI1+ME-cases that carried cryptic 3q26 breakpoints, which were not found in the EVI1+ME+group. EVI1+ME-expression predicts an extremely poor prognosis distinguishable from the general EVI1+AML patients (overall survival [OS]: P < .001 and event-free survival [EFS]: P = .002). We argue that EVI1/ME quantitative expression analysis should be implemented in the molecular diagnostic procedures of AML.
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|Persistent URL||dx.doi.org/10.1182/blood-2007-10-119230, hdl.handle.net/1765/28804|
Lugthart, S., van Drunen, E., van Norden, Y., van Hoven, A., Erpelinck, C.A.J., Valk, P.J.M., … Delwel, H.R.. (2008). High EVI1 levels predict adverse outcome in acute myeloid leukemia: Prevalence of EVI1 overexpression and chromosome 3q26 abnormalities underestimated. Blood, 111(8), 4329–4337. doi:10.1182/blood-2007-10-119230