In paraneoplastic neurological syndromes associated with Hu-antibodies (Hu-PNS) an important role for cellular immunity is hypothesized. We characterized the cerebrospinal fluid (CSF) pleocytosis in Hu-PNS patients by assessing the major lymphocyte subsets by flow cytometry. The B cell subset in the CSF of Hu-PNS patients showed a significant absolute (~ 20×) and relative (~ 3×) expansion, while the numbers of CD4+ T cells, CD8+ T cells and NK cells only showed an absolute expansion (~ 4-7×) compared to the controls. On the other hand, the NKT cell subset showed a significant relative reduction in CSF and in blood of Hu-PNS patients. The relative B cell expansion is consistent with the intrathecal synthesis of Hu-antibodies, while the increased number of T and NK cells supports an additional role for cellular immunity in the pathogenesis of Hu-PNS. In addition, the autoimmune hypothesis of Hu-PNS is supported by the relative NKT cell deficiency.

Additional Metadata
Keywords Cancer, Cerebrospinal fluid, Hu-antibody, Lymphocyte subsets, Paraneoplastic neurological syndromes
Persistent URL dx.doi.org/10.1016/j.jneuroim.2008.01.007, hdl.handle.net/1765/29282
Citation
de Graaf, M.T., de Beukelaar, J.W.K., Bergsma, J., Kraan, J., van den Bent, M.J., Klimek, M., … Gratama, J.W.. (2008). B and T cell imbalances in CSF of patients with Hu-antibody associated PNS. Journal of Neuroimmunology, 195(1-2), 164–170. doi:10.1016/j.jneuroim.2008.01.007