Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups.
The UV-induced unscheduled DNA synthesis (UDS) in cultured cells of excision-deficient xeroderma pigmentosum (XP) complementation groups A through I was assayed after injection of Micrococcus luteus UV-endonuclease using glass microneedles. In all complementation groups a restoration of the UV-induced UDS, in some cells to the repair-proficient human level, was observed. Another prokaryotic DNA-repair enzyme, T4 endonuclease V, restored the UV-induced UDS in a similar way after microinjection into XP cells. Since both enzymes specifically catalyse only the incision of UV-irradiated DNA, we conclude that this activity is impaired in cells of all 9 excision-deficient XP complementation groups tested.
|Keywords||*DNA Repair, *N-Glycosyl Hydrolases, 0 (Multienzyme Complexes), 81611-73-6 (UV endonuclease), 9007-49-2 (dna), Cells, Cultured, DNA/biosynthesis/*radiation effects, EC 3.1.- (Endodeoxyribonucleases), EC 188.8.131.52 (deoxyribonuclease V), EC 3.2.2.- (N-Glycosyl Hydrolases), Endodeoxyribonucleases/administration & dosage, Genetic Complementation Test, Human, Micrococcus/enzymology, Microinjections, Multienzyme Complexes/*administration & dosage, Support, Non-U.S. Gov't, Ultraviolet Rays, Xeroderma Pigmentosum/enzymology/*genetics|
|Persistent URL||dx.doi.org/10.1016/0027-5107(85)90106-X, hdl.handle.net/1765/2983|
de Jonge, A.J.R., Vermeulen, W., Keijzer, W., Hoeijmakers, J.H.J., & Bootsma, D.. (1985). Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups.. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 150(1-2), 99–105. doi:10.1016/0027-5107(85)90106-X