1985
Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups.
Publication
Publication
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis , Volume 150 - Issue 1-2 p. 99- 105
The UV-induced unscheduled DNA synthesis (UDS) in cultured cells of excision-deficient xeroderma pigmentosum (XP) complementation groups A through I was assayed after injection of Micrococcus luteus UV-endonuclease using glass microneedles. In all complementation groups a restoration of the UV-induced UDS, in some cells to the repair-proficient human level, was observed. Another prokaryotic DNA-repair enzyme, T4 endonuclease V, restored the UV-induced UDS in a similar way after microinjection into XP cells. Since both enzymes specifically catalyse only the incision of UV-irradiated DNA, we conclude that this activity is impaired in cells of all 9 excision-deficient XP complementation groups tested.
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| , , , , , , , , , , , , , , , , , , | |
| doi.org/10.1016/0027-5107(85)90106-X, hdl.handle.net/1765/2983 | |
| Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis | |
| Organisation | Erasmus MC: University Medical Center Rotterdam |
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de Jonge, A. J. R., Vermeulen, W., Keijzer, W., Hoeijmakers, J., & Bootsma, D. (1985). Microinjection of Micrococcus luteus UV-endonuclease restores UV-induced unscheduled DNA synthesis in cells of 9 xeroderma pigmentosum complementation groups. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 150(1-2), 99–105. doi:10.1016/0027-5107(85)90106-X |
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