The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae.
The RAD6 gene of Saccharomyces cerevisiae encodes a ubiquitin conjugating enzyme and is required for DNA repair, DNA-damage-induced mutagenesis and sporulation. Here, we show that RAD6 and the rhp6+ gene from the distantly related yeast Schizosaccharomyces pombe share a high degree of structural and functional homology. The predominantly acidic carboxyl-terminal 21 amino acids present in the RAD6 protein are absent in the rhp6(+)-encoded protein; otherwise, the two proteins are very similar, with 77% identical residues. Like rad6, null mutations of the rhp6+ gene confer a defect in DNA repair, UV mutagenesis and sporulation, and the RAD6 and rhp6+ genes can functionally substitute for one another. These observations suggest that functional interactions between RAD6 (rhp6+) protein and other components of the DNA repair complex have been conserved among eukaryotes.
|Keywords||0 (DNA, Fungal), 0 (Fungal Proteins), 0 (RAD6 protein, S cerevisiae), 0 (rhp6 protein, S pombe), Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA Repair/genetics, DNA, Fungal/genetics, EC 6. (Ligases), EC 18.104.22.168 (Ubiquitin-Conjugating Enzymes), Fungal Proteins/*genetics, Genetic Complementation Test, Ligases/*genetics, Molecular Sequence Data, Mutation, Saccharomyces cerevisiae/*genetics, Saccharomycetales/*genetics, Schizosaccharomyces/*genetics, Sequence Homology, Nucleic Acid, Spores, Fungal/genetics, Support, Non-U.S. Gov't, Support, U.S. Gov't, P.H.S., Ubiquitin-Conjugating Enzymes, Ultraviolet Rays|
Reynolds, P., Koken, M.H.M., Hoeijmakers, J.H.J., Prakash, S., & Prakash, L.. (1990). The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae.. EMBO Journal, 9, 1423–1430. Retrieved from http://hdl.handle.net/1765/3018