Induction of neutralizing antibody in mice against poliovirus type II with monoclonal anti-idiotypic antibody.
Syngeneic monoclonal anti-idiotope antibody Ab2,2-17C3SCC was raised against an idiotope on a protective monoclonal antibody with specificity for poliovirus type II. Ab2,2-17C3SCC detects a paratope-related interspecies IdX. Ab2,2-17C3SCC purified from supernatant fluids of hybridoma cells by protein A-Sepharose was injected into 4- to 6-wk-old BALB/c mice. The sera of the mice were screened for the expression of antibodies bearing the corresponding idiotope. Immunization of mice with Ab2,2-17C3SCC induced antibodies of complementary specificity. Furthermore, micro VN tests suggest that Ab2,2-17C3SCC can substitute for antigen in the induction of anti-polio neutralizing antibodies, and hence can function as a monoclonal anti-idiotypic vaccine.
|Keywords||0 (Antibodies, Monoclonal), 0 (Antibodies, Viral), 0 (Antigens, Viral), 0 (Immunoglobulin Idiotypes), Animals, Antibodies, Monoclonal/*administration & dosage/analysis/biosynthesis, Antibodies, Viral/*biosynthesis, Antigen-Antibody Reactions, Antigens, Viral/immunology, Binding, Competitive, Cross Reactions, Immunization, Passive, Immunoglobulin Idiotypes/*immunology, Mice, Mice, Inbred BALB C, Neutralization Tests, Polioviruses/*immunology|
Uytdehaag, F.G.C.M., & Osterhaus, A.D.M.E.. (1985). Induction of neutralizing antibody in mice against poliovirus type II with monoclonal anti-idiotypic antibody.. Journal of Immunology, 134(2), 1225–1229. Retrieved from http://hdl.handle.net/1765/3292