Human peripheral blood lymphocytes from recently vaccinated individuals produce both type-specific and intertypic cross-reacting neutralizing antibody on in vitro stimulation with one type of poliovirus.
An in vitro system of poliovirus-specific antibody production by peripheral blood B cells on stimulation by the virus has been developed. Virus-neutralizing antibodies in culture supernatant fluids, or virus-specific antibody-secreting cells (ASC) were detected by microneutralization assay and ELISA-SPOT test, respectively. After booster immunization with polio vaccine, anti-poliovirus-neutralizing ASC were present in circulation. This response was measurable between 5 and 12 days after booster vaccination. At between 12 and 90 days, another subset of B cells was found in peripheral blood that only produced poliovirus-specific neutralizing antibody after in vitro antigenic stimulation. The in vitro virus-induced response required B cells, monocytes, and T4+ (T helper) cells, and was shown to result from de novo protein synthesis. The anti-poliovirus-neutralizing response in vitro could be dissected in a type-specific and intertypic cross-reactive response by using various antigen concentrations for in vitro stimulation. Evidence was obtained by absorption studies for the existence of intertypic cross-reactive neutralization-inducing epitopes.
|Keywords||*Antibody Specificity, *Lymphocyte Activation, 0 (Antibodies, Viral), 0 (Diphtheria Toxoid), 0 (Diphtheria-Tetanus-Pertussis Vaccine), 0 (Drug Combinations), 0 (Pertussis Vaccine), 0 (Poliovirus Vaccine, Inactivated), 0 (Tetanus Toxoid), Adolescent, Adult, Antibodies, Viral/*biosynthesis/classification, B-Lymphocytes/classification/immunology/*metabolism, Cross Reactions, Diphtheria Toxoid/administration & dosage, Diphtheria-Tetanus-Pertussis Vaccine, Drug Combinations/administration & dosage, Human, Kinetics, Male, Neutralization Tests, Pertussis Vaccine/administration & dosage, Poliovirus Vaccine, Inactivated/*administration & dosage/immunology, T-Lymphocytes, Helper-Inducer/immunology, Tetanus Toxoid/administration & dosage, Time Factors|
Uytdehaag, F.G.C.M., Loggen, H.G., Logtenberg, T., Lichtveld, R.A., van Steenis, G., van Asten, J.A.A.M., & Osterhaus, A.D.M.E.. (1985). Human peripheral blood lymphocytes from recently vaccinated individuals produce both type-specific and intertypic cross-reacting neutralizing antibody on in vitro stimulation with one type of poliovirus.. Journal of Immunology, 35(5), 3094–3101. Retrieved from http://hdl.handle.net/1765/3302