Comparison of an enzyme-linked immunosorbent assay, an immunofluorescence assay and a hemagglutination inhibition assay for detection of antibodies to K-papovavirus in mice.
The sensitivity of a newly developed enzyme-linked immunosorbent assay (ELISA) for detection of antibody to K virus was compared with the sensitivities of an immunofluorescence assay (IFA) and a hemagglutination inhibition assay (HIA). Specific pathogen-free BALB/c RIVM mice, 5 weeks old, were inoculated intraperitoneally with a mouse organ suspension containing 10(4.5) TCID50 of K virus per dose. Control animals were inoculated with a control mouse organ suspension. No clinical signs were observed during the 7 weeks they were followed for the development of serum antibody. The ELISA proved to be the most sensitive of the three assays and demonstrated K virus-specific antibodies as early as 3 days after infection.
|Keywords||*Polyomaviridae, 0 (Antibodies, Viral), Animals, Antibodies, Viral/*analysis, Comparative Study, Enzyme-Linked Immunosorbent Assay, Fluorescent Antibody Technique, Hemagglutination Inhibition Tests, Mice, Mice, Inbred BALB C, Papillomaviridae/*immunology, Predictive Value of Tests, Specific Pathogen-Free Organisms, Tumor Virus Infections/*immunology|
Groen, J., Osterhaus, A.D.M.E., Broeders, H.W.J., & Spijkers, H.E.M.. (1989). Comparison of an enzyme-linked immunosorbent assay, an immunofluorescence assay and a hemagglutination inhibition assay for detection of antibodies to K-papovavirus in mice.. Laboratory Animal Science, 39(1), 21–24. Retrieved from http://hdl.handle.net/1765/3356