Measles virus-specific murine T cell clones: characterization of fine specificity function.
Measles virus (MV)-specific murine helper T cell clones (Thy-1.2+, CD4+, CD8-) were generated from mice immunized with MV-infected mouse brain homogenate by limiting dilution and in vitro stimulation of spleen cells with UV-inactivated MV Ag. The protein specificity of 7 out of 37 stable T cell clones, which displayed MHC-restricted MV Ag recognition, could be assessed by using purified MV proteins. Two fusion (F) protein-specific, two hemagglutinin-specific, and three nucleoprotein- or matrix protein-specific clones were shown to be established. The F protein-specific T cell clones together with a panel of previously generated F protein-specific T cell clones were characterized for their fine specificity by using beta-galactosidase fusion products, which contained different parts of the F protein. It was shown that at least two epitopes on the major part of the F protein (amino acid 2-513) can be recognized by mouse T cells. Functional characterization of three T cell clones showed that they were able to assist MV-specific B cells and bystander B cells for antibody production. Furthermore, they were shown to produce the lymphokines IL-2 and IFN-gamma. It was also shown that these T cell clones induced a MV-specific delayed type hypersensitivity response. These observations suggest that all of the T cell clones characterized belong to the TH1 helper subset.
|Keywords||0 (Viral Fusion Proteins), Animals, B-Lymphocytes/immunology, Clone Cells, Hypersensitivity, Delayed, Measles virus/*immunology, Mice, Mice, Inbred BALB C, Support, Non-U.S. Gov't, T-Lymphocytes, Helper-Inducer/classification/*immunology, Viral Fusion Proteins/immunology|
de Vries, P., Versteeg-van Oosten, J.P.M., Visser, I.K.G., van Binnendijk, R.S., Langeveld, S.A., Osterhaus, A.D.M.E., & Uytdehaag, F.G.C.M.. (1989). Measles virus-specific murine T cell clones: characterization of fine specificity function.. Journal of Immunology, 142(8), 2841–2846. Retrieved from http://hdl.handle.net/1765/3363