Rabies virus-specific human T cell clones provide help for an in vitro antibody response against neutralizing antibody-inducing determinants of the viral glycoprotein.
Human T cell clones were prepared from peripheral blood mononuclear cells from a vaccinated human donor and kept in culture in the presence of rabies virus antigen and growth factors. Phenotypic analysis of the T cell clones revealed expression of the CD3 and CD4 cell surface markers, but not of CD8, consistent with a phenotype of helper/inducer T cells. The rabies virus specificity of the T cell clones was established by virus-specific proliferation in response to the rabies virus Pitman-Moore strain (PM) produced in three different cell substrates. The clones also responded to the rabies virus strains Evelyn-Rokitnicki-Abelseth (ERA) and challenge virus standard (CVS), but not to the rabies virus-related Mokola and Duvenhage-6 virus strains. Proliferative responses of T cell clones required rabies virus antigen to be presented by autologous antigen-presenting cells in association with HLA class II molecules. When cultured with rabies virus antigen, but in the absence of growth factors, some of the T cell clones provided help for an antibody response of rabies virus immune B lymphocytes. Analysis of culture supernatant fluids showed that at least a part of this antibody response was directed against neutralizing antibody-inducing determinants of the viral glycoprotein.
|Keywords||0 (Antibodies, Viral), 0 (Antigens, Viral), 0 (Epitopes), 0 (Glycoproteins), 0 (Rabies Vaccines), 0 (Viral Proteins), Antibodies, Viral/analysis/*biosynthesis, Antigens, Viral/*immunology, Binding, Competitive, Clone Cells/immunology, Epitopes/immunology, Glycoproteins/immunology, Human, Major Histocompatibility Complex, Neutralization Tests, Phenotype, Rabies Vaccines/administration & dosage/immunology, Rabies virus/*immunology, T-Lymphocytes, Helper-Inducer/*immunology, Viral Proteins/*immunology|
Bunschoten, H., Klapmuts, R.J., Claassen, I.J.Th.M., Reijneveld, S.D., Osterhaus, A.D.M.E., & Uytdehaag, F.G.C.M.. (1989). Rabies virus-specific human T cell clones provide help for an in vitro antibody response against neutralizing antibody-inducing determinants of the viral glycoprotein.. Journal of General Virology, 70, 1513–1521. Retrieved from http://hdl.handle.net/1765/3364