Delineation of canine parvovirus T cell epitopes with peripheral blood mononuclear cells and T cell clones from immunized dogs.
Three synthetic peptides derived from the amino acid sequence of VP2 of canine parvovirus (CPV) which were recently shown to represent three distinct T cell epitopes for BALB/c mice could prime BALB/c mice for a CPV-specific proliferative T cell response upon immunization. Proliferative responses of peripheral blood mononuclear cells (PBMC) from CPV-immunized dogs upon stimulation with these and other peptides, covering the major part of the sequence of VP2', identified the presence of T cell epitopes for this species. Most of these epitopes were recognized by PBMC from only a minority of the dogs tested. With three newly generated canine Thyl+ T cell clones, which recognized CPV antigen in association with major histocompatibility complex class II molecules, two distinct T cell epitopes were identified within the unique sequence of VP1.
|Keywords||0 (Epitopes), 0 (Peptides), 0 (Viral Proteins), Amino Acid Sequence, Animals, Capsid/*immunology, Clone Cells, Dogs/*immunology/microbiology, Epitopes, Leukocytes, Mononuclear/*immunology, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Parvoviridae/*genetics, Peptides/immunology, Support, Non-U.S. Gov't, T-Lymphocytes, Helper-Inducer/immunology, T-Lymphocytes/*immunology, Viral Proteins/*immunology|
Rimmelzwaan, G.F., Poelen, M.C.M., Meloen, R.H., Carlson, J., Uytdehaag, F.G.C.M., & Osterhaus, A.D.M.E.. (1990). Delineation of canine parvovirus T cell epitopes with peripheral blood mononuclear cells and T cell clones from immunized dogs.. Journal of General Virology, 71, 2321–2329. Retrieved from http://hdl.handle.net/1765/3408