Measles virus transmembrane fusion protein synthesized de novo or presented in iscom is endogenously processed for HLA class I- and class II-restricted cytotoxic T cell recognition.
The routes used by antigen-presenting cells (APC) to convert the transmembrane fusion glycoprotein (F) of measles virus (MV) to HLA class I and class II presentable peptides have been examined, using cloned cytotoxic T lymphocytes in functional assays. Presentation by Epstein-Barr virus-transformed B lymphoblastoid cell lines was achieved using live virus, ultraviolet light-inactivated virus, and purified MV-F delivered either as such or incorporated in immunostimulating complexes (MV-F-ISCOM). Only live virus and MV-F-ISCOM allow presentation by class I molecules, while all antigen preparations permit class II-restricted presentation. We observe presentation of MV-F from live virus and as MV-F-ISCOM by class II molecules in a fashion that is not perturbed by chloroquine. Our studies visualize novel presentation pathways of type I transmembrane proteins.
|Keywords||*Lymphocyte Activation, 0 (Antigens, CD4), 0 (Antigens, CD8), 0 (Histocompatibility Antigens Class I), 0 (Histocompatibility Antigens Class II), 0 (Viral Fusion Proteins), Animals, Antigen-Presenting Cells/physiology, Antigens, CD4/analysis, Antigens, CD8/analysis, Histocompatibility Antigens Class I/*immunology, Histocompatibility Antigens Class II/*immunology, Human, Measles virus/*immunology, Support, Non-U.S. Gov't, T-Lymphocytes, Cytotoxic/*immunology, Viral Fusion Proteins/*biosynthesis/immunology|
van Binnendijk, R.S., van Baalen, C.A., Poelen, M.C.M., de Vries, P., Boes, J., Cerundolo, V., … Uytdehaag, F.G.C.M.. (1992). Measles virus transmembrane fusion protein synthesized de novo or presented in iscom is endogenously processed for HLA class I- and class II-restricted cytotoxic T cell recognition.. The Journal of Experimental Medicine, 176, 119–128. Retrieved from http://hdl.handle.net/1765/3445