Human HLA class I- and HLA class II-restricted cloned cytotoxic T lymphocytes identify a cluster of epitopes on the measles virus fusion protein.
The transmembrane fusion (F) glycoprotein of measles virus is an important target antigen of human HLA class I- and class II-restricted cytotoxic T lymphocytes (CTL). Genetically engineered F proteins and nested sets of synthetic peptides spanning the F protein were used to determine sequences of F recognized by a number of F-specific CTL clones. Combined N- and C-terminal deletions of the respective peptides revealed that human HLA class I and HLA class II-restricted CTL efficiently recognize nonapeptides or decapeptides representing epitopes of F. Three distinct sequences recognized by three different HLA class II (DQw1, DR2, and DR4/w53)-restricted CTL clones appear to cluster between amino acids 379 and 466 of F, thus defining an important T-cell epitope area of F. Within this same region, a nonamer peptide of F was found to be recognized by an HLA-B27-restricted CTL clone, as expected on the basis of the structural homology between this peptide and other known HLA-B27 binding peptides.
|Keywords||0 (Epitopes), 0 (HLA-D Antigens), 0 (Histocompatibility Antigens Class I), 0 (Peptides), 0 (Recombinant Fusion Proteins), 0 (Viral Fusion Proteins), Amino Acid Sequence, Clone Cells, Comparative Study, Epitopes, HLA-D Antigens/*immunology, Histocompatibility Antigens Class I/*immunology, Human, Immunity, Cellular, Measles virus/*immunology, Molecular Sequence Data, Peptides/chemistry/immunology, Recombinant Fusion Proteins/immunology, Sequence Alignment, Sequence Homology, Amino Acid, Support, Non-U.S. Gov't, T-Lymphocytes, Cytotoxic/*immunology, Viral Fusion Proteins/*immunology|
van Binnendijk, R.S., Versteeg-van Oosten, J.P.M., Poelen, M.C.M., Brugghe, H.F., Hoogerhout, P., Osterhaus, A.D.M.E., & Uytdehaag, F.G.C.M.. (1993). Human HLA class I- and HLA class II-restricted cloned cytotoxic T lymphocytes identify a cluster of epitopes on the measles virus fusion protein.. Journal of Virology, 67, 2276–2284. Retrieved from http://hdl.handle.net/1765/3461