Viral replication and development of specific immunity in macaques after infection with different measles virus strains.
Cynomolgus monkeys (Macaca fascicularis) were experimentally infected with a wild type measles virus (MV) strain (MV-BIL). Following intratracheal inoculation with different infectious doses, the virus could be isolated from peripheral blood mononuclear cells (PBMC), lung lavage cells, and pharyngeal cells. The kinetics of the cell-associated viremia was similar in all infected animals. They developed specific serum IgM, IgG, and neutralizing antibody responses as well as MV-specific T cell-mediated immunity. Monkeys infected intratracheally or intramuscularly with the wild type MV-Edmonston or the attenuated MV-Schwartz strain showed a lower level of PBMC-associated viremia and less pronounced specific IgM responses. Nine months after infection with MV strains, all of the monkeys were protected from intratracheal reinfection with MV-BIL. This monkey model is suitable for study of new generations of vaccines and vaccination strategies for measles.
|Keywords||*Disease Models, Animal, *Macaca fascicularis, *Virus Replication, 0 (Antibodies, Viral), 0 (Immunoglobulin G), 0 (Immunoglobulin M), Animals, Antibodies, Viral/biosynthesis, Human, Immunity, Cellular, Immunoglobulin G/biosynthesis, Immunoglobulin M/biosynthesis, Kinetics, Leukocytes, Mononuclear/microbiology, Lung/cytology/microbiology, Measles virus/immunology/*physiology, Measles/immunology/*microbiology, Pharynx/cytology/microbiology, Support, Non-U.S. Gov't, T-Lymphocytes/immunology, Viremia/immunology/microbiology|
van Binnendijk, R.S., van der Heijden, R.W.J., van Amerongen, G., Uytdehaag, F.G.C.M., & Osterhaus, A.D.M.E.. (1994). Viral replication and development of specific immunity in macaques after infection with different measles virus strains.. The Journal of Infectious Diseases, 70, 443–448. Retrieved from http://hdl.handle.net/1765/3503