BACKGROUND. Specificity of transgene expression is important for safety during gene therapeutical applications. For prostate cancer, transcriptional targeting has been applied but was hampered by loss of specificity and low activity. We constructed a small chimeric promoter for high and prostate-specific transgene expression from adenoviral vectors. METHODS. A chimeric promoter, composed of the prostate-specific antigen (PSA) enhancer and the rat probasin promoter, was cloned into an adenoviral vector and its activity was compared to vectors containing conventional prostate-specific promoters and the constitutive Cytomegalovirus (CMV) promoter in in vitro and in vivo prostate cancer models. RESULTS. The chimeric PSA-probasin promoter was the most active prostate-specific promoter reaching up to 20% of CMV promoter activity while maintaining prostate-specificity. CONCLUSIONS. The chimeric PSA-probasin promoter is a small promoter that can be utilized in viral vectors for high prostate-specific transgene expression.

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Keywords Adenovirus, Gene therapy, Mouse model, Prostate cancer, Transcriptional targeting
Persistent URL dx.doi.org/10.1002/pros.20560, hdl.handle.net/1765/35945
Citation
Kraaij, R, van der Weel, L, Ridder, C.M.A, van der Korput, H.A.G.M, Zweistra, J.L.M, Rijswijk, A.L, … Trapman, J. (2007). A small chimeric promoter for high prostate-specific transgene expression from adenoviral vectors. The Prostate, 67(8), 829–839. doi:10.1002/pros.20560