CD8+ cytotoxic T lymphocytes of a cynomolgus macaque infected with simian immunodeficiency virus (SIV)mac32H-J5 recognize a nine amino acid epitope in SIV Gag p26.
A detailed analysis of simian immunodeficiency virus (SIV)-specific cytotoxic T lymphocyte (CTL) responses and the identification of the proteins and epitopes they target may improve the design of immunotherapeutic interventions and provide insights into AIDS pathogenesis. Here, we identified a new CTL epitope in the SIV Gag protein, recognized by CD8+ and MHC class I-restricted CTL clones from a long-term asymptomatic cynomolgus macaque (Macaca fascicularis) infected with SIVmac32H-J5. Using overlapping synthetic peptides, the optimal minimal epitope was characterized as a nine amino acid peptide representing amino acids 242-250 of p26 (SVDEQIQWM). CTL recognition was shown to be abolished by amino acid substitutions observed within homologous human immunodeficiency virus (HIV)-1 and HIV-2 sequences.
|Keywords||0 (Antigens, Viral), 0 (Epitopes), 0 (Gene Products, gag), Animals, Antigens, Viral/*immunology, CD8-Positive T-Lymphocytes/*immunology/virology, Epitopes/immunology, Gene Products, gag/*immunology, Human, Immunity, Cellular, Macaca fascicularis/immunology/*virology, SIV/*immunology, Simian Acquired Immunodeficiency Syndrome/*immunology|
Geretti, A.M., Hulskotte, E.G.J., Dings, M.E.M., van Baalen, C.A., van Amerongen, G., & Osterhaus, A.D.M.E.. (1997). CD8+ cytotoxic T lymphocytes of a cynomolgus macaque infected with simian immunodeficiency virus (SIV)mac32H-J5 recognize a nine amino acid epitope in SIV Gag p26.. Journal of General Virology, 78, 821–824. Retrieved from http://hdl.handle.net/1765/3595