Objective: A previous study in piglets with experimental pneumonia showed that reducing atelectasis by means of open lung ventilation attenuated bacterial translocation compared to conventional ventilation settings. This study examined the effect of open lung ventilation with higher than necessary positive end-expiratory pressures (PEEP) on bacterial translocation. Design and setting: Prospective animal study in a university-affiliated research laboratory. Subjects: Thirty piglets. Interventions: Animals were surfactant-depleted by whole-lung lavage and infected with group B streptococci. Thereafter the animals were ventilated for 5 h according to either a conventional ventilation strategy, open lung strategy, or open lung/high-PEEP strategy. Blood samples for blood gas analysis and blood bacterial counts were taken every hour. After 5 h of ventilation surviving animals were killed, and lung colony forming units and lung mechanics parameters were determined. Results: All animals in both open lung groups survived but only 30% of those in the conventional ventilation group. Open lung ventilation resulted in significantly less bacterial translocation than either conventional or high-PEEP ventilation. Lung function in the conventional ventilated group was significantly less than in the two open lung groups. Conclusions: The lowest level of bacterial translocation was observed during optimal ventilation (open lung) which was achieved by using individually tailored settings. Deviation to either side can be harmful, as shown by the increased bacterial translocation during conventional and high-PEEP ventilation.

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Keywords Atelectasis, Lavage, Open-lung, Pneumonia, Positive end-expiratory pressure, Ventilation
Persistent URL dx.doi.org/10.1007/s00134-007-0749-1, hdl.handle.net/1765/36020
Lachmann, R.A., van Kaam, A.H., & Haitsma, J.J.. (2007). High positive end-expiratory pressure levels promote bacterial translocation in experimental pneumonia. Intensive Care Medicine, 33(10), 1800–1804. doi:10.1007/s00134-007-0749-1