A subtype-specific peptide-based enzyme immunoassay for detection of antibodies to the G protein of human respiratory syncytial virus is more sensitive than routine serological tests.
Peptides deduced from the central conserved region (residues 158 to 189) of protein G of human respiratory syncytial virus (HRSV) subtypes A and B were used as antigens in subtype-specific enzyme-linked immunosorbent assays (G-peptide ELISAs). These G-peptide ELISAs were compared with seven other serological assays to detect HRSV infection: ELISAs based on complete protein G, on fusion protein F, and on nucleoprotein N; a complement fixation assay; a virus neutralization test; and ELISAs for the detection of immunoglobulin A (IgA) or IgM antibodies specific for HRSV. In paired serum samples from patients with HRSV infection, more infections were diagnosed by the G-peptide ELISA (67%) than by all other serological tests combined (48%). Furthermore, for 16 of 18 patients (89%), the G-peptide ELISAs were able to differentiate between antibodies against HRSV subtypes A and B. This study shows that peptides corresponding to the central conserved region of the attachment protein G of HRSV can successfully be used as antigens in immunoassays. The G-peptide ELISA appeared to be more sensitive than conventional tests for the detection of HRSV antibody titer rises.
|Keywords||*HN Protein, 0 (Antibodies, Viral), 0 (Antigens, Viral), 0 (HN Protein), 0 (RSV proteins, Respiratory syncytial virus), 0 (Viral Proteins), 0 (attachment protein G), Amino Acid Sequence, Antibodies, Viral/*analysis/immunology, Antigens, Viral/immunology, Enzyme-Linked Immunosorbent Assay/*methods, Human, Infant, Infant, Newborn, Molecular Sequence Data, Respiratory Syncytial Virus Infections/*immunology/virology, Respiratory Syncytial Viruses/*immunology, Sensitivity and Specificity, Viral Proteins/*immunology|
Langedijk, J.P.M., Brandenburg, A.H., Middel, W.G.J., Osterhaus, A.D.M.E., Meloen, R.H., & van Oirschot, J.T.. (1997). A subtype-specific peptide-based enzyme immunoassay for detection of antibodies to the G protein of human respiratory syncytial virus is more sensitive than routine serological tests.. Journal of Clinical Microbiology, 35(7), 1656–1660. Retrieved from http://hdl.handle.net/1765/3604