Validation of an HIV-1 inactivation protocol that is compatible with intracellular drug analysis by mass spectrometry
Mass spectrometry is a powerful tool for studying the intracellular pharmacokinetics of antiretroviral drugs. However, the biohazard of HIV-1 calls for a safety protocol for such analyses. To this end, we extracted HIV-1 producing cells with methanol or ethanol at 4 °C. After extraction, no viral infectivity was detected, as shown by a reduction in infectious titers of more than 6 log. In addition, this protocol is compatible with the quantitative analysis of antiretroviral drugs in cell extracts using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. Thus, using this protocol, infectious HIV-1 is inactivated and antiretroviral drugs are extracted from cells in a single step.
|Keywords||Antiviral, HIV, Inactivation, Infectious, Intracellular, MALDI|
|Persistent URL||dx.doi.org/10.1016/j.jchromb.2006.10.001, hdl.handle.net/1765/37060|
van Kampen, J.J.A, Verschuren, E.J, Burgers, P.C, Luider, T.M, de Groot, R, Osterhaus, A.D.M.E, & Gruters, R.A. (2007). Validation of an HIV-1 inactivation protocol that is compatible with intracellular drug analysis by mass spectrometry. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 847(1), 38–44. doi:10.1016/j.jchromb.2006.10.001