Broadening of coreceptor usage by human immunodeficiency virus type 2 does not correlate with increased pathogenicity in an in vivo model.
The pathogenic properties of four primary human immunodeficiency virus type 2 (HIV-2) isolates and two primary HIV-2 biological clones were studied in an in vivo human-to-mouse chimeric model. The cell-associated viral load and the ability to reduce the severity of the induced graft-versus-host disease symptoms, the CD4/CD8 ratio and the level of repopulation of the mouse tissues by the graft, were determined. All HIV-2 strains, irrespective of their in vitro biological phenotype, replicated to high titres and significantly reduced graft-versus-host disease symptoms as well as the CD4/CD8 ratios. Reduction of graft repopulation caused by infection with the respective HIV-2 strains showed that the in vitro replication rate, syncytium-inducing capacity and ability to infect human macrophages did influence the in vivo pathogenic potential whereas broadening of coreceptor usage did not.
|Keywords||Acute Disease, Animals, CD4-CD8 Ratio, Chimera, Disease Models, Animal, Graft vs Host Disease/immunology, HIV Infections/*etiology/immunology/virology, HIV-2/*pathogenicity/physiology, Humans, Immunohistochemistry, Leukocytes, Mononuclear/transplantation, Mice, Receptors, HIV/*physiology, Transplantation, Heterologous, Virus Replication|
van der Ende, M.E., Guillon, C., Boers, P.H.M., Gruters, R.A., Racz, P., Tenner-Racz, K., … Schutten, M.. (2000). Broadening of coreceptor usage by human immunodeficiency virus type 2 does not correlate with increased pathogenicity in an in vivo model.. Journal of General Virology, 81(2), 507–513. Retrieved from http://hdl.handle.net/1765/3710