Vaccination with rabies to study the humoral and cellular immune response to a T-cell dependent neoantigen in man
We investigated the humoral (antigen-specific immunoglobulin isotypes, IgG subclasses, and avidity maturation) and cellular (antigen-specific in vitro proliferation) immune response in 18 healthy adult volunteers, following a primary and a single booster vaccination with the T-cell dependent neoantigen rabies administered at a 3-months interval. The IgG antibody titer showed a mean 31-fold increase (range 3–154) 4 weeks after the first vaccination and a memory response was observed after booster vaccination, i.e. high IgG titers, switch from IgM to IgG and IgA and increased antibody avidity. All healthy adults showed a rabies-induced proliferative response with a mean stimulation index of 45 (range 3.5–200) after in vitro stimulation of PBMC obtained at 4 weeks after booster vaccination. The results obtained in this study provide a frame of reference for the interpretation of specific immune responses to the T-cell dependent neoantigen rabies in patients suspected of a primary or secondary immunodeficiency. Humoral and cellular immune responses to the rabies neoantigen provide complementary information on the condition of the immune system of an individual. Five patients diagnosed with a combined immunodeficiency were vaccinated using the same protocol and showed a number of abnormalities, either in the humoral or the cellular immune response to the rabies neoantigen.
|Keywords||antibody response, immunodeficiency, in vitro proliferation, neoantigen, rabies|
|Persistent URL||dx.doi.org/JOCI.0000010429.36461.6b, hdl.handle.net/1765/3936|
Brinkman, D.M., ten Dam, M.M., Vossen, J.M.J.J., Osterhaus, A.D.M.E., Kroon, F.P., van Tol, M.J., & Jol-van der Zijde, C.M.. (2003). Vaccination with rabies to study the humoral and cellular immune response to a T-cell dependent neoantigen in man. Journal of Clinical Immunology, 23(6), 528–538. doi:JOCI.0000010429.36461.6b