HIV-2-Infected Individuals With Undetectable Plasma Viremia Carry Replication-Competent Virus in Peripheral Blood Lymphocytes
Using an optimized HIV co-culture protocol it was possible to isolate infectious HIV-2 variants from 6 HIV-2-infected individuals who had undetectable plasma viremia and maintained high CD4+ T-cell numbers for prolonged periods. This shows for the first time that HIV-2-infected individuals with no demonstrable in vivo virus production carry replication-competent virus in peripheral blood mononuclear cells (PBMCs). The frequency of PBMCs with infectious virus was low, ranging from 0.01–0.9 infectious units per million (IUPM) CD4+ T cells with a median value of 0.2 IUPM. In comparison, viremic HIV-2-infected individuals had a 2-log higher median infectious load (36 IUPM, range 1–673; P = 0.003). HIV-2 infectious load correlated with CD4 counts (rs = -0.88, P < 0.0001). The low infectious load in aviremic HIV-2-infected persons is reminiscent of what has been observed for HIV-1 infection controlled by highly active antiretroviral therapy.
|Keywords||HIV-2, aviremic HIV-2 infection, infectious cellular load, peripheral blood mononuclear cells, plasma RNA load, replication-competent virus|
|Persistent URL||dx.doi.org/10.1097/00126334-200407010-00003, hdl.handle.net/1765/3955|
Blaak, H., Boers, P.H.M., Schutten, M., van der Enden, M.E., & Osterhaus, A.D.M.E.. (2004). HIV-2-Infected Individuals With Undetectable Plasma Viremia Carry Replication-Competent Virus in Peripheral Blood Lymphocytes. J A I D S, 36(3), 777–782. doi:10.1097/00126334-200407010-00003