Model systems to study biochemical functions of rat sertoli cells

Development of roale genn cells (spermatogenesis) takes place in the testicular seminiferous tubules. The honrones follicle-stimulating honnone (FSH) and testosterone are essential for the initiation and maintenance of spermatogenesis. n>e genn cells in the tubules (spermatogonia, spermatocytes and spermatids; in order of development) are in intimate contact with the Sertoli cells which are attached to a basal lamina. Adjacent Sertoli cells are connected through tight junctions, thus foming a sertoli cell barrier. The barrier divides the tubules in a basal and an adlum:inal c:anparbnent. Gem cells enter the adluminal compartment when they have reached a certain stage of development, and are thereafter not in direct contact with the environment outside the tubules. Consequently, Sertoli cell secretion products are thought to play an :inportant role during spermatogenesis. sertoli cells contain FSH and testosterone receptors 1 and are target cells for these homones. The germ cells, however, do not contain receptors for FSH and testosterone. Honnonal control of spermatogenesis therefore involves effects of hormones on Sertoli cells. However, the peritubular cells, surrounding the tubules, may also take part in this hormonal regulation.