The aim of the work described in this thesis is to gain more insight in the role of the Cockayne Syndrome A (GSA) and B (CSB) proteins in the process of transcriptioncoupled DNA repair and transcription. Using biochemical analysis and live cell studies we investigated the molecular behavior of both proteins. First, we analyzed the chromatin remodeling activity of CSB. Secondly, using green fluorescent protein (GFP) technology (Chapter 4) and photobleaching we studied (i) the dynamic behavior of CSB in TCR and transcription in living cells, (ii) the kinetics of GSA in TCR and its molecular connection with the CSB protein and (iii) the cellular localization of CSB with respect to other nuclear processes like transcription and mRNA processing.

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Erasmus University Rotterdam
J.H.J. Hoeijmakers (Jan)
hdl.handle.net/1765/51350
Erasmus MC: University Medical Center Rotterdam

van den Boom, V. (2003, December 17). Dynamic organization of transcription-coupled DNA repair. Retrieved from http://hdl.handle.net/1765/51350