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I. Skachkov (Ilya), Y. Luan (Ying), S.T. van Tiel (Sandra), A.F.W. van der Steen (Ton), K. Kooiman (Klazina), M.R. Bernsen (Monique) and N. de Jong (Nico)

2012-12-01

Ultrasound contrast agents mediated cell labeling for MRI tracking

Publication

Publication

In vivo cell tracking is an emerging field and a promising technique for tracking stem cells, metastasis and inflammation. The aim of this study was to find optimal US parameters for ultrasound contrast agent-mediated SPIO-labeling of endothelial cells for MRI tracking. The optimal ultrasound parameters were 40 kPa at 1 MHz, 10,000 cycles, 20 Hz pulse repetition frequency, applied 5 minutes after SPIO addition. This gave a ∼7 times increase of SPIO uptake in endothelial cells in vitro compared to the control with 90% cell viability. This technique could therefore be safely used for labeling of endothelial cells with SPIO for MRI tracking.

Additional Metadata
Keywords cell tracking, MRI, SPIO, targeted microbubbles, ultrasound contrast agents
Persistent URL doi.org/10.1109/ULTSYM.2012.0569, hdl.handle.net/1765/54452
Organisation Department of Cardio-Thoracic Surgery
Citation
Skachkov, I., Luan, Y., van Tiel, S., van der Steen, T., Kooiman, K., Bernsen, M., & de Jong, N. (2012). Ultrasound contrast agents mediated cell labeling for MRI tracking. doi:10.1109/ULTSYM.2012.0569


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