2013-08-02
Locus-specific proteomics by TChP: Targeted chromatin purification
Publication
Publication
Cell Reports , Volume 4 - Issue 3 p. 589- 600
Here, we show that transcription factors bound to regulatory sequences can be identified by purifying these unique sequences directly from mammalian cells invivo. Using targeted chromatin purification (TChP), a double-pull-down strategy with a tetracycline-sensitive "hook" bound to a specific promoter, we identify transcription factors bound to the repressed γ-globin gene-associated regulatory regions. After validation of the binding, we show that, in human primary erythroid cells, knockdown of a number of these transcription factors induces γ-globin gene expression. Reactivation of γ-globin gene expression ameliorates the symptoms of β-thalassemia and sickle cell disease, and these factors provide potential targets for the development of therapeutics for treating these patients
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doi.org/10.1016/j.celrep.2013.07.004, hdl.handle.net/1765/57141 | |
Cell Reports | |
Organisation | Department of Cardio-Thoracic Surgery |
Pourfarzad, F., Aghajanirefah, A., deBoer, E., TenHave, S., BrynvanDijk, T., Kheradmandkia, S., … Grosveld, F. (2013). Locus-specific proteomics by TChP: Targeted chromatin purification. Cell Reports, 4(3), 589–600. doi:10.1016/j.celrep.2013.07.004 |