1995-08-01
Cre-mediated site-specific translocation between nonhomologous mouse chromosomes
Publication
Publication
Proceedings of the National Academy of Sciences of the United States of America , Volume 92 - Issue 16 p. 7376- 7380
Chromosome rearrangements, such as large deletions, inversions, or translocations, mediate migration of large DNA segments within or between chromosomes, which can have major effects on cellular genetic control. A method for chromosome manipulation would be very useful for studying the consequences of large-scale DNA rearrangements in mammalian cells or animals. With the use of the Cre-loxP recombination system of bacteriophage P1, we induced a site-specific translocation between the Dek gene on chromosome 13 and the Can gene on chromosome 2 in mouse embryonic stem cells. The estimated frequency of Cre-mediated translocation between the nonhomologous mouse chromosomes is approximately 1 in 1200-2400 embryonic stem cells expressing Cre recombinase. These results demonstrate the feasibility of site-specific recombination systems for chromosome manipulation in mammalian cells in vivo, breaking ground for chromosome engineering.
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| doi.org/10.1073/pnas.92.16.7376, hdl.handle.net/1765/57265 | |
| Proceedings of the National Academy of Sciences of the United States of America | |
| Organisation | Department of Biochemistry |
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van Deursen, J., Fornerod, M., van Rees, B., & Grosveld, G. (1995). Cre-mediated site-specific translocation between nonhomologous mouse chromosomes. Proceedings of the National Academy of Sciences of the United States of America, 92(16), 7376–7380. doi:10.1073/pnas.92.16.7376 |
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