2005-01-31
X chromosome choice occurs independently of asynchronous replication timing
Publication
Publication
The Journal of Cell Biology , Volume 168 - Issue 3 p. 365- 373
In mammals, dosage compensation is achieved by X chromosome inactivation in female cells. Xist is required and sufficient for X inactivation, and Xist gene deletions result in completely skewed X inactivation. In this work, we analyzed skewing of X inactivation in mice with an Xist deletion encompassing sequence 5 KB upstream of the promoter through exon 3. We found that this mutation results in primary nonrandom X inactivation in which the wild-type X chromosome is always chosen for inactivation. To understand the molecular mechanisms that affect choice, we analyzed the role of replication timing in X inactivation choice. We found that the two Xist alleles and all regions tested on the X chromosome replicate asynchronously before the start of X inactivation. However, analysis of replication timing in cell lines with skewed X inactivation showed no preference for one of the two Xist alleles to replicate early in S-phase before the onset of X inactivation, indicating that asynchronous replication timing does not play a role in skewing of X inactivation.
Additional Metadata | |
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doi.org/10.1083/jcb.200405117, hdl.handle.net/1765/59383 | |
The Journal of Cell Biology | |
Organisation | Biophysical Genomics, Department Cell Biology & Genetics |
Gribnau, J., Luikenhuis, S., Hochedlinger, K., Monkhorst, K., & Jaenisch, R. (2005). X chromosome choice occurs independently of asynchronous replication timing. The Journal of Cell Biology, 168(3), 365–373. doi:10.1083/jcb.200405117 |