2004-07-01
In vitro susceptibilities of Madurella mycetomatis to itraconazole and amphotericin B assessed by a modified NCCLS method and a viability-based 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl] -2H-tetrazolium hydroxide (XTT) assay
Publication
Publication
Antimicrobial Agents and Chemotherapy , Volume 48 - Issue 7 p. 2742- 2746
Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5- [(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of the isolates tested are inhibited [MIC 50]) of 0.06 to 0.13 mg/liter) was superior to amphotericin B (MIC50 of 0.5 to 1.0 mg/liter).
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| doi.org/10.1128/AAC.48.7.2742-2746.2004, hdl.handle.net/1765/60045 | |
| Antimicrobial Agents and Chemotherapy | |
| Organisation | Department of Medical Microbiology and Infectious Diseases |
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Ahmed, A., van de Sande, W., van Vianen, W., van Belkum, A., Fahal, A., Verbrugh, H., & Bakker-Woudenberg, I. (2004). In vitro susceptibilities of Madurella mycetomatis to itraconazole and amphotericin B assessed by a modified NCCLS method and a viability-based 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl] -2H-tetrazolium hydroxide (XTT) assay. Antimicrobial Agents and Chemotherapy, 48(7), 2742–2746. doi:10.1128/AAC.48.7.2742-2746.2004 |
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