2006-08-01
An Unexpected Role for IL-3 in the Embryonic Development of Hematopoietic Stem Cells
Publication
Publication
Developmental Cell , Volume 11 - Issue 2 p. 171- 180
Cytokines are important in adult hematopoiesis, yet their function in embryonic hematopoiesis has been largely unexplored. During development, hematopoietic stem cells (HSCs) are found in the aorta-gonad-mesonephros (AGM) region, yolk sac (YS), and placenta and require the Runx1 transcription factor for their normal generation. Since IL-3 is a Runx1 target and this cytokine acts on adult hematopoietic cells, we examined whether IL-3 affects HSCs in the mouse embryo. Using Runx1 haploinsufficient mice, we show that IL-3 amplifies HSCs from E11 AGM, YS, and placenta. Moreover, we show that IL-3 mutant embryos are deficient in HSCs and that IL-3 reveals the presence of HSCs in the AGM and YS prior to the stage at which HSCs are normally detected. Thus, our studies support an unexpected role for IL-3 during development and strongly suggest that IL-3 functions as a proliferation and/or survival factor for the earliest HSCs in the embryo.
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| doi.org/10.1016/j.devcel.2006.07.002, hdl.handle.net/1765/60878 | |
| Developmental Cell | |
| Organisation | Biophysical Genomics, Department Cell Biology & Genetics |
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Robin, C., Ottersbach, K., Durand, C., Peeters, M., Vanes, L., Tybulewicz, V., & Dzierzak, E. (2006). An Unexpected Role for IL-3 in the Embryonic Development of Hematopoietic Stem Cells. Developmental Cell, 11(2), 171–180. doi:10.1016/j.devcel.2006.07.002 |
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