2012-06-01
CD49f-based selection of circulating tumor cells (CTCs) improves detection across breast cancer subtypes
Publication
Publication
Cancer Letters , Volume 319 - Issue 1 p. 49- 55
Circulating tumor cells (CTCs) can be enumerated using CellSearch, but not all breast cancer subtypes, specifically those with epithelial-mesenchymal transition (EMT) characteristics, sufficiently express the enrichment (EpCAM) and selection (CK8/18/19) markers used in this method. While CD146 can detect EpCAM-negative CTCs, we here evaluated the value of various cytokeratins and CD49f to detect CK8/18/19-negative CTCs. The tested cytokeratins provided no substantial benefit, but adding CD49f to CK8/18/19 as a selection marker resulted in improved recovery of normal-like cell lines.Combined staining of CK8/18/19 and CD49f after CD146/EpCAM enrichment is likely to further improve CTC detection in breast cancer.
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| doi.org/10.1016/j.canlet.2011.12.031, hdl.handle.net/1765/65283 | |
| Cancer Letters | |
| Organisation | Department of Medical Oncology |
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Mostert, B., Kraan, J., Sieuwerts, A., van der Spoel, P., Bolt-de Vries, J., Prager-van der Smissen, W., … Sleijfer, S. (2012). CD49f-based selection of circulating tumor cells (CTCs) improves detection across breast cancer subtypes. Cancer Letters, 319(1), 49–55. doi:10.1016/j.canlet.2011.12.031 |
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