1994-12-01
Detection of Bacteroides fragilis endotoxin in amniotic fluid by counterimmunoelectrophoresis
Publication
Publication
Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology , Volume 66 - Issue 4 p. 333- 336
The ability of counter immunoelectrophoresis (CIE) to detectBacteroides fragilis endotoxin in amniotic fluid in small concentrations was evaluated. A method was developed which, in combination with ultrafiltration, permits detection ofB. fragilis endotoxin in amniotic fluid in a concentration of 40 ng/ml or more. The sensitivity threshold was reduced to 2 ng/ml by using a highly reactive IgG-fraction isolated from rabbit anti-B. fragilis IPL E 323 antiserum.
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| doi.org/10.1007/BF00882769, hdl.handle.net/1765/65872 | |
| Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology | |
| Organisation | Department of Gynaecology & Obstetrics |
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Beckmann, I., de Graaff, K., Meisel-Mikolajczyk, F., & Wallenburg, H. (1994). Detection of Bacteroides fragilis endotoxin in amniotic fluid by counterimmunoelectrophoresis. Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 66(4), 333–336. doi:10.1007/BF00882769 |
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