Tissue specific and androgen-regulated expression of human prostate-specific transglutaminase
Transglutaminases (TGases) are calcium-dependent enzymes catalysing the post-translational cross-linking of proteins. In the prostate at least two TGases are present, the ubiquitously expressed tissue-type TGase (TGC), and a prostate-restricted TGase (TGP). This paper deals with the molecular cloning and characterization of the cDNA encoding the human prostate TGase (hTGP). For this purpose we have screened a human prostate cDNA library with a probe from the active-site region of TGC. The largest isolated cDNA contained an open reading frame encoding a protein of 684 amino acids with a predicted molecular mass of 77 kDa as confirmed by in vitro transcription-translation and subsequent SDS/PAGE. The hTGP gene was tissue-specifically expressed in the prostate, yielding an mRNA of approx. 3.5 kb. Furthermore, a 3-fold androgen-induced upregulation of hTGP mRNA expression has been demonstrated in the recently developed human prostate cancer cell line, PC346C. Other well established human prostate cancer cell lines, LNCaP and PC-3, showed no detectable hTGP mRNA expression on a Northern bolt. The gene coding for prostate TGase was assigned to chromosome 3.
|Keywords||Amino Acid Sequence, Androgens/*metabolism, Animals, Base Sequence, Chromosome Mapping, Chromosomes, Human, Pair 3/genetics, Cloning, Molecular, Cricetinae, DNA Probes/genetics, DNA, Complementary/genetics, Female, Gene Expression Regulation, Enzymologic, Humans, Hybrid Cells, Male, Mice, Molecular Sequence Data, Prostate/*enzymology, Prostatic Neoplasms/enzymology/genetics, Sequence Homology, Amino Acid, Tissue Distribution, Transglutaminases/genetics/*metabolism, Tumor Cells, Cultured|
Dubbink, H.J., Verkaik, N.S., Faber, P.W., Trapman, J., Schröder, F.H., & Romijn, J.C.. (1996). Tissue specific and androgen-regulated expression of human prostate-specific transglutaminase. Biochemical Journal. Retrieved from http://hdl.handle.net/1765/8612