Molecular typing of Salmonella typhi strains from Dhaka (Bangladesh) and development of DNA probes identifying plasmid-encoded multidrug-resistant isolates
Seventy-eight Salmonella typhi strains isolated in 1994 and 1995 from patients living in Dhaka, Bangladesh, were subjected to phage typing, ribotyping, IS200 fingerprinting, and PCR fingerprinting. The collection displayed a high degree of genetic homogeneity, because restricted numbers of phage types and DNA fingerprints were observed. A significant number of the S. typhi strains (67%) were demonstrated to be multiple drug resistant (MDR). The vast majority of the MDR strains were resistant to chloramphenicol, ampicillin, trimethoprim, streptomycin, sulfamethoxazole, and tetracycline (R type CATmSSuT), a resistance phenotype that has also frequently been observed in India. Only two strains displayed a distinct MDR phenotype, R type AT-mSSuT. Pulsed-field gel electrophoresis demonstrated the presence of large plasmids exclusively in the MDR strains of both R types. The plasmids present in the S. typhi strains of R type CATmSSuT could be conjugated to Escherichia coli and resulted in the complete transfer of the MDR phenotype. PCR fingerprinting allowed discrimination of MDR and susceptible strains. The DNA fragments enabling discrimination of MDR and susceptible S. typhi strains by PCR were useful genetic markers for identifying MDR encoded by large plasmids of the H1 incompatibility group.
|Keywords||*Bacterial Typing Techniques, Bangladesh/epidemiology, Base Sequence, Child, DNA Fingerprinting/methods, DNA Probes, DNA, Bacterial/genetics/isolation & purification, Drug Resistance, Multiple/genetics, Epidemiology, Molecular, Evaluation Studies, Genotype, Humans, Phenotype, Plasmids/genetics, Polymerase Chain Reaction/methods, Polymorphism, Genetic, Salmonella typhi/*classification/drug effects/*genetics, Typhoid Fever/epidemiology/microbiology|
Hermans, P.W.M., Saha, S.K., van Leeuwen, W.J., Verbrugh, H.A., van Belkum, A.F., & Goessens, W.H.F.. (1996). Molecular typing of Salmonella typhi strains from Dhaka (Bangladesh) and development of DNA probes identifying plasmid-encoded multidrug-resistant isolates. Journal of Clinical Microbiology. Retrieved from http://hdl.handle.net/1765/8616