The transcription factor GATA6 is essential for early extraembryonic development
The gene coding for the murine transcription factor GATA6 was inactivated by insertion of a beta-galactosidase marker gene. The analysis of heterozygote GATA6/lacZ mice shows two inductions of GATA6 expression early in development. It is first expressed at the blastocyst stage in part of the inner mass and in the trophectoderm. The second wave of expression is in parietal endoderm (Reichert's membrane) and the mesoderm and endoderm that form the heart and gut. Inactivation leads to a lethality shortly after implantation (5.5 days postcoitum). Chimeric experiments show this to be caused by an indirect effect on the epiblast due to a defect in an extraembryonic tissue.
|Keywords||*Gene Expression Regulation, Developmental, Animals, Blastocyst/metabolism, Chimera, Crosses, Genetic, DNA-Binding Proteins/*genetics/*metabolism, Embryonic and Fetal Development, Female, GATA6 Transcription Factor, Genes, Reporter, Gestational Age, Male, Mice, Mice, Inbred C57BL, Mice, Transgenic, Recombinant Fusion Proteins/biosynthesis, Research Support, Non-U.S. Gov't, Restriction Mapping, Transcription Factors/*genetics/*metabolism, Transfection, Zinc Fingers, beta-Galactosidase/genetics/metabolism|
Koutsourakis, M., Langeveld, A., Patient, R., Beddington, R., & Grosveld, F.G.. (1999). The transcription factor GATA6 is essential for early extraembryonic development. Development (Cambridge). Retrieved from http://hdl.handle.net/1765/9125