Multilocus sequence typing of Staphylococcus aureus with DNA array technology
July 2003
Article
volume 41, issue 7 pp 3323-3326.
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A newly developed oligonucleotide array suited for multilocus sequence typing (MLST) of Staphylococcus aureus strains was analyzed with two strain collections in a two-center study. MLST allele identification for the first strain collection fully agreed with conventional strain typing. Analysis of strains from the second collection revealed that chip-defined MLST was concordant with conventional MLST. Array-mediated MLST data were reproducible, exchangeable, and epidemiologically concordant.
Keywords
- Humans
- Oligonucleotide Array Sequence Analysis/*methods
- Alleles
- Bacterial Typing Techniques
- *Sequence Analysis, DNA
- Bacterial Proteins/*genetics
- Methicillin Resistance
- Staphylococcal Infections/microbiology
- Staphylococcus aureus/*classification/drug effects/*genetics
Automatically Extracted Terms
- 2-2-2-2-6-3-2
- strain
- sequence
- center
- aureus
- 10-14-8-6-10-3-2
- array
- allele
- probe
- 3-3-1-12-4-4-16
- typing
- collection
- staphylococcus
- result
- staphylococcus aureus strains
- genechip
- microbiol
- table
- multilocus sequence typing
- method