Intracellularly expressed single-domain antibody against p15 matrix protein prevents the production of porcine retroviruses
January 2003
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The presence of porcine endogenous retroviruses presents a potential risk of transmission of infectious diseases (xenozoonosis) if tissues and organs from genetically modified pigs are to be used in xenotransplantation. Here, we report that intracellular expression of a llama single-domain antibody against p15, the matrix domain protein of the porcine endogenous retrovirus Gag polyprotein, blocks retrovirus production, providing the possibility of eliminating the risk of infection in xenotransplantation.
Keywords
- Animals
- Humans
- Research Support, Non-U.S. Gov't
- Amino Acid Sequence
- Molecular Sequence Data
- Epitope Mapping
- Virus Replication
- Immune Sera/immunology
- Antibodies, Viral/*physiology
- Antibody Affinity
- Endogenous Retroviruses/*physiology
- Gene Products, gag/*antagonists & inhibitors/immunology
- Immunoglobulin Heavy Chains/physiology
- Immunoglobulin Variable Region/physiology
- Retroviridae Infections/*prevention & control
- Swine/*virology
- Transplantation, Heterologous/*adverse effects
- Viral Matrix Proteins/*antagonists & inhibitors/immunology
Automatically Extracted Terms
- antibody
- protein
- pk 15 cells
- virus
- clone
- retrovirus
- expression
- porcine
- gag protein
- 5 vhh
- production
- rt activity
- llama
- matrix
- induction
- fragment
- activity
- perv-b
- particle
- tet-on
