http://hdl.handle.net/1765/10325

Preferential HLA usage in the influenza virus-specific CTL response


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To study whether individual HLA class I alleles are used preferentially or equally in human virus-specific CTL responses, the contribution of individual HLA-A and -B alleles to the human influenza virus-specific CTL response was investigated. To this end, PBMC were obtained from three groups of HLA-A and -B identical blood donors and stimulated with influenza virus. In the virus-specific CD8(+) T cell population, the proportion of IFN-gamma- and TNF-alpha-producing cells, restricted by individual HLA-A and -B alleles, was determined using virus-infected C1R cells expressing a single HLA-A or -B allele for restimulation of these cells. In HLA-B*2705- and HLA-B*3501-positive individuals, these alleles were preferentially used in the influenza A virus-specific CTL response, while the contribution of HLA-B*0801 and HLA-A*0101 was minor in these donors. The magnitude of the HLA-B*0801-restricted response was even lower in the presence of HLA-B*2705. C1R cells expressing HLA-B*2705, HLA-A*0101, or HLA-A*0201 were preferentially lysed by virus-specific CD8(+) T cells. In contrast, the CTL response to influenza B virus was mainly directed toward HLA-B*0801-restricted epitopes. Thus, the preferential use of HLA alleles depended on the virus studied.



Keywords


Automatically Extracted Terms
  • hla-a
  • influenza
  • response
  • c 1r cells
  • donor
  • virus-specific
  • hla-b
  • virus
  • allele
  • epitope
  • b alleles
  • group
  • hla-matched blcl cells
  • virus-specific ctl response
  • stimulation
  • target cells
  • hla usage
  • percentage
  • expression
  • virus-specific ctl


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