Chromatin interaction mechanism of transcriptional control in vivo.
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We have used a kinetic analysis to distinguish possible mechanisms of activation of transcription of the different genes in the human beta globin locus. Based on in situ studies at the single-cell level we have previously suggested a dynamic mechanism of single genes alternately interacting with the locus control region (LCR) to activate transcription. However, those steady-state experiments did not allow a direct measurement of the dynamics of the mechanism and the presence of loci with in situ primary transcript signals from two beta-like genes in cis has left open the possibility that multiple genes in the locus could initiate transcription simultaneously. Kinetic assays involving removal of a block to transcription elongation in conjunction with RNA FISH show that multiple beta gene primary transcript signals in cis represent a transition between alternating transcriptional periods of single genes, supporting a dynamic interaction mechanism.
- Research Support, Non-U.S. Gov't
- Molecular Sequence Data
- Base Sequence
- Mice, Transgenic
- Nucleic Acid Synthesis Inhibitors/pharmacology
- Cells, Cultured
- In Situ Hybridization, Fluorescence
- Binding, Competitive/genetics
- Locus Control Region/drug effects
- Transcription, Genetic/drug effects/*physiology