Accuracy of the VITEK 2 system to detect glycopeptide resistance in enterococci.
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We evaluated the accuracy of the VITEK 2 fully automated system to detect and identify glycopeptide-resistant enterococci (GRE) compared to a reference agar dilution method. The sensitivity of vancomycin susceptibility testing with VITEK 2 for the detection of vanA, vanB, and vanC1 strains was 100%. The sensitivity of vancomycin susceptibility testing of vanC2 strains was 77%. The sensitivity of teicoplanin susceptibility testing of vanA strains was 90%. Of 80 vanC enterococci, 78 (98%) were correctly identified by VITEK 2 as Enterococcus gallinarum/Enterococcus casseliflavus. Since the identification and susceptibility data are produced within 3 and 8 h, respectively, VITEK 2 appears a fast and reliable method for detection of GRE in microbiology laboratories.
- Sensitivity and Specificity
- Anti-Bacterial Agents/*pharmacology
- Drug Resistance, Microbial/genetics
- Microbial Sensitivity Tests/*methods
- Bacterial Proteins/genetics
- Enterococcus/*drug effects/genetics
- Gram-Positive Bacterial Infections/*microbiology
- Vancomycin Resistance/genetics