Functional transformation of the chromatoid body in mouse spermatids requires testis-specific serine/threonine kinases
February 2010
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The cytoplasmic chromatoid body (CB) organizes mRNA metabolism and small regulatory RNA pathways, in relation to haploid gene expression, in mammalian round spermatids. However, little is known about functions and fate of the CB at later steps of spermatogenesis, when elongating spermatids undergo chromatin compaction and transcriptional silencing. In mouse elongating spermatids, we detected accumulation of the testis-specific serine/threonine kinases TSSK1 and TSSK2, and the substrate TSKS, in a ring-shaped structure around the base of the flagellum and in a cytoplasmic satellite, both corresponding to structures described to originate from the CB. At later steps of spermatid differentiation, the ring is found at the caudal end of the newly formed mitochondrial sheath. Targeted deletion of the tandemly arranged genes Tssk1 and Tssk2 in mouse resulted in male infertility, with loss of the CB-derived ring structure, and with elongating spermatids possessing a collapsed mitochondrial sheath. These results reveal TSSK1- and TSSK2-dependent functions of a transformed CB in post-meiotic cytodifferentiation of spermatids.
- article
- female
- male
- priority journal
- controlled study
- animal experiment
- animal tissue
- enzyme activity
- mouse
- nonhuman
- cell differentiation
- unclassified drug
- cell transformation
- animal cell
- gene deletion
- Kinase
- cellular distribution
- protein serine threonine kinase
- cell loss
- Spermatogenesis
- enzyme substrate
- mitochondrion
- chromatid
- spermatid
- spermatogenesis
- Chromatoid body
- Mitochondrial sheath
- Mouse
- Nuage
- Spermatids
- cell structure
- cytoplasm
- enzyme localization
- enzyme structure
- chromosome satellite
- male infertility
- spermatozoon tail
- testis specific serine threonine kinase 1
- testis specific serine threonine kinase 2
